Předmět Environmental Analytical Chemistry - Mgr. (ACA06E)

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Materiál	Typ	Datum	Počet stažení

Cíl

Graduates in agricultural engineering will not work as analytical chemists in analytical laboratories. However, they will frequently send their samples to such laboratories and use analytical data produced by these laboratories. Hence, they should be fully qualified partners to the analytical chemists. In sampling part, they will have even greater responsibility then analytical chemist. Further considerations include the quality of analytical result, its information content, price for analysis etc. The course intends not to educate future personnel of analytical labs, but rather a fully qualified partners to the chemical analysts sharing their deal of responsibility, who are also able to interpret the analytical result in proper way. Particular attention is paid to the entire sampling-analytical system, with measuring step only as one (though important) part of it. Theoretical lectures are complemented with practical laboratory excercises in small groups of 3-5 students working in professional laboratories of our Faculty. Students thus have an access to the best instrumentation available in the building, and a chance to discuss all aspects of analyses with their personnel. The course is concluded by the excursion visit to some professional analytical enterprise in Prague.

Osnova

PřednáškaIntroductionGravimetryRedox titrationAbsorption of lightDetermination of selected metals (Fe, Mn, Ca, Mg) in ecosystemsOptical methods II (molecular absorption, emission and fluorescence spectrometry)Electroanalytical methods IElectroanalytical methods IISeparation methods ISeparation methods II (liquid chromatography) HPLCSeparation methods III (gel electrophoresis, isotachophoresis, sedimentation techniques)Hyphenated methodsAbiotic and biotic stress factors and their influence on secondary polyphenolic metabolitesData evaluationCvičení Manganometry and chelatometryChelatometry and iodometryMolecular spectrometry (UV-VIS)Atomic absorption spectrometry - F-AAS, ETA-AASElectrochemistryDetermination of nitrates by ISEDetermination of Hg by AMA-254Polarography - determination of ascorbic acid on Eco-Tribo Polarosensor polarographGas chromatography - principles, example of separation of natural compoundsGC separation and determination of selected pesticides (triazines, etcLiquid chromatographySeparation of components of plant extracts by SEC according to molecular weightsGel electrophoresisHyphenated techniques, e

Získané způsobilosti

Znalosti:Students are provided with a general good knowledge of understanding of environmental analytical chemistry and of qualitative, quantitative and instrumental methods used in the determination of analytes and environmental pollutants. They acquire knowledge of the methods of gravimetric, volumetric, acid-base titration, redox and chelatometric titration, optical methods (atomic and molecular absorption and emission spectroscopy - AAS, FAAS, etc.), and electrochemical methods (polarography, voltametry, conductometry, electrophoresis), they are aware of the principles of separation methods (extraction, chromatography). They are familiar with the principles and practical arrangement of hyphenated systems, the GS-MS and HPLC-DAD-UV-VIS and HPLC-ESI-MS, etc. They know the application of these methods for determination of individual analytes. Students possess knowledge of the application of detectors on different principles - electrochemical and optical - refractometer, UV-VIS, DAD, etc. They know the principle of specific analytical instruments used in trace analysis, such as the AMA for Hg determination in various materials of biological origin. They can classify various pollutants in the environment and also the environmental hazards.Dovednosti:Students are able to perform analytical techniques (gravimetry, voltametry, molecular spectroscopy) and to provide instrumental separation techniques (GC, HPLC). They implement the chemical applications in solving practical problems in analytical chemistry. They are able to select appropriate analytical and sampling system for determination of selected analytes and implement it according to standard operating procedures. They are able to produce a report determining the outcome of individual obtained results. Students know how to creatively evaluate the suitability of individual methods and their financial performance, accuracy, reproducibility and time required for each analysis. Students are able to orient themselves in Special Analytical Laboratories - GC, HPLC, micro-laboratories. Students can independently identify the different groups of environmental pollutants and they acquire knowledge of their determination. Students are provided with a detailed overview of the analytical procedures used for determining. They are able to assess the suitability of different analytical procedures for other related subjects (such as Environmental chemistry, etc.) and management specializing in the environment.Kompetence - komunikace:Students are able to work closely with their superiors. They are able to act within their professional skills in the English language and specific terminology. They can communicate on a professional level with specialists in the field of analytics and communicatively link environmental issues and natural resources with special analytical procedures. They can formulate their own views, as well as activities assigned to staff.Kompetence - úsudek:Students are able to plan their activities and also manage the activities of other working team members. They are able to work with information sources in the English language (databases, websites, literature and other documents) and apply them to professional and scientific research in the unified form in the frame of this subject and evenly related subjects.

Literatura

ZákladníChuráček, J. 1993. Advanced Instrumental Methods of Chemical Analysis. Academia, Praha, 486 pp. ISBN 3-540-58508-7.Eisler, R. 1995. Contaminant Hazard Reviews. U.S. Department of the Interior U.S. Geological Survey. Patuxent Wildlife Research Center, CD.Harvey, D. 2000. Modern Analytical Chemistry. Mc Graw Hill, London, 816 pp. ISBN 0-07-237547-7.Lachman, J., Mader, P. 2003. Selected Chapters from Environmental Analytical Chemistry. AF CUA Prague, CD.Manahan, S.E. 2001. Environmental Chemistry. 7th ed. Boca Raton, CRC Press LLC, London. ISBN 1-56670-492-8. CD.Salomons, W., Förstner, U., Mader, P. 1995. Heavy Metals Problems and Solutions. Springer Verlag, Berlin, 412 pp. ISBN 3-540-58508-7.DoporučenáBicchi, C., Sandra, P. 1987. Capillary Gas Chromatography in Essential Oil Analysis. Heidelberg, Huthig, 435 pp. ISBN 3-7785-0860-1.Bauwell, C.N. 1994. Fundamentals of Molecular Spectroscopy. London, Mc Graw-Hill, 300 pp. ISBN 0-07-707976-0.Giddings, J.C., Grushka, E., Brown, P.R. 1987. Advances in Chromatography. N.Y. Marcel Dekker, 359 pp. ISBN 0-8247-7770-0.Ho, M.H. 1990. Analytical Methods in Forensic Chemistry. New York, London, Ellis Horwood, 400 pp. ISBN-10-0130379611.Karasek, F.W., Clement, R.E. 1988. Basic Gas Chromatography - Mass Spectrometry. Amsterdam, Elsevier Science, 201 pp. ISBN 0-444-42760-0.Mikeš, O. 1988. High-performance Liquid Chromatography of Biopolymers and Biooligomers. Amsterdam, Elsevier, 380 pp. ISBN 0-444-42951-4.Pacáková, V., Štulík, K. 1990. High Performance Liquid Chromatography. Praha, SPN, 138 pp. ISBN 80-7066-203-4.Svanberg, S. 1992. Atomic and Molecular Spectroscopy. Berlin, Springer, 405 pp. ISBN 0-387-55243-X.Wenclawiak, B. 1992. Analysis with Supercritical Fluids Extraction and Chromatography. Berlin, Springer, 213 pp. ISBN 3-540-55420-3.Wendisch, D.A.W. 1990. Acronyms and Abbreviations in Molecular Spectroscopy. Berlin, Springer, 315 pp. ISBN 0-387-51348-5.

Požadavky

none

Garant

prof. Ing. Jaromír Lachman, CSc.