Předmět General Molecular Biology - Mgr. (AGI05E)

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Materiál	Typ	Datum	Počet stažení

Osnova

PřednáškaThe basic dogma of molecular geneticsNucleic acids as carriers of genetic informationGeneral principles of DNA replicationClassification of viruses and viroids by molecular biologyProkaryotic replicationEukaryotic replicationProkaryotic transcriptionEukaryotic transcriptionProkaryotic translationEukaryotic translationExpression of prokaryotic genomeExpression of eukaryotic genomeMolecular-genetic view of the eukaryotic cell cycleHistorical overview of basic discoveries in molecular geneticsCvičeníPrinciples of work in molecular genetics laboratoryBiological material for the isolation of nucleic acids, fixation and preservation of the materialIsolation of nucleic acids of viral originReverse transcription of viral RNA and its useIsolation of bacterial chromosomal and plasmid DNAMethods for separation of bacterial plasmids based on ultracentrifugationIsolation of plant nuclear DNA by various laboratory proceduresPurification of isolated genomic DNAPurification of genomic DNA isolated based on ultracentrifugationQuantification of nucleic acidsMethods for visualization of nucleic acidsElectrophoretic separation of DNADNA digestionBioinformatics

Získané způsobilosti

Znalosti:Graduate has complete information in the field of applied molecular biology necessary for next specialisation in the field of gene technology and biotechnology. He knows structure of nucleic acids and understands to the biochemical principle of genetic code. He is able to characterise cells of prokaryotes and eukaryotes in the view of primary dogma of molecular genetics. He has a good knowledge about organisation of cellular genome and genome of non-cellular particles (virusoids, viroids and viruses). He can describe the structure of non-coding regions of eukaryotic genome including the transposable elements. He is able in detail characterise mechanism and enzymatic apparatus of DNA replication and mechanisms of its regulation. He can characterise replication errors and its connections to point mutations. Student knows in detail processes of transcription and translation. He can characterise gene expression regulation at levels of transcription, translation and posttranscriptional and posttranslational modifications. He has overview of connections between molecular biology and evolutionary biology. Practically, he has detailed knowledge of analytical methods and bioinformatic databases generally used in molecular biology.Dovednosti:Graduate has very good skills necessary for independent work in laboratories aimed on molecular biology. He knows principles of safety and ethic of work and is able them independently apply. He knows principles of biohazard waste safe disposal. He is able use precision analytical methods used in molecular biology. He copes with work in sterile conditions of flow-box. He can choose suitable biological material for optimal extraction of nucleic acids. Biological material he can adequately fix and conserve it for long time. He independently works with freeze-dryer and liquid nitrogen. He can choose suitable method for nucleic acids extraction, independently prepares required solutions and buffers. He has skills in application of different type of fragmentation analyses. He can separate nucleic acids or proteins by means the agarose or polyacrylamide electrophoresis and capillary electrophoresis. He has overview of methods of electrophoreograms visualisation and archiving. He can evaluate the quantity and quality of extracted nucleic acids. Graduate can independently work with nucleotide databases and uses range of bioinformatic tools.Kompetence - komunikace:Graduate is able of independent activity and activity in team. He can very good communicate in a sphere of fundamental and also applied research. He is able of communication in Czech and foreign language. He uses technical terminology typical for molecular biology applied in the field of applied research and breeding.Kompetence - úsudek:Graduate creates his opinions on the basis of wide range of information resources. He is able these resources analyse and process. He uses synthesis and deduction. In the work he is initiative and active. He can define work objectives and generalises obtained results. He is able classify and evaluate results and conclusions of scientific research. He is able responsibly manage activity of team members.

Literatura

ZákladníGlick, B. R., Thompson, J. E. 1993. Methods in plant molecular biology and biotechnology. CRC Press. London. p. 360. ISBN 0849351642.Henry, R. J. 1997. Practical applications of plant molecular biology. Chapman & Hall. London. p. 258. ISBN 0412732203.Brown, T. A. 1999. Genomes. Bios Sci. Publ. Oxford. UK. p. 608. ISBN 9780471316183.Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. 2003. Essential Cell Biology (2nd Ed.). W. H. Freeman & Co. New York. USA. p. 740. ISBN 081533480X.DoporučenáBrooker, R. J. 2012. Genetics Analysis and principles. McGraw Hill. New York. p. 860. ISBN 9780073525280.Snustad, D. P., Simmons, M. J. 2011. Principles of Genetics. John Wiley & Sons. Hoboken. USA. p. 766. ISBN 0470903597.

Požadavky

Žádné

Garant

doc. Dr. Ing. Pavel Vejl, Ph.D.