Předmět Bioinorg. Chem. & Biophys. of Plants (KEBR / 203)

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Materiál	Typ	Datum	Počet stažení

Obsah

Content of lectures Some lectures marked * may still be changed depending on the availability of guest lecturersWeek Days General Theme Lectures1 Mon Introduction into themes of this course Introduction:Bioinorganic chemistry & Biophysics of Plants"Tue Biophysics of Photosynthesis as the fundamental process in plantsWed Basics,selected important methods, with emphasis on applications in bioinorganic chemistry and biophysics of plants, and special emphasis on methods for analysis in vivo, in situ and methods developed or improved in the Küpper group (e.g. FKM, GPS-fitting, QISH) Methods for analysing photosynthesis (fluorescence kinetics, absorption changes, O2/CO2 measurements)Thu Further methods of UV-VIS absorption and fluorescence spectroscopy in vivo and in situFri Work with metalloproteins: expression, isolation, purification and characterisation by biochemical assays and metalloproteomics2 Mon Basics of coordination chemistry in biological SystemsTue * Magnetic Resonance Spectroscopy (EPR, NMR) as structural toolsWed X-ray emission spectroscopy (EDX, PIXE, ?XRF)for analysing element distribution in biological samples and X-ray absorption spectroscopy (XANES, EXAFS) for analysing biological metal complexesThu Chromatographic methods: basics, advanced HPLC methodsFri Metal uptake and transport, Metals as essential elements (incl. metal deficiency stress) Trace element nutrition in plants: uptake and transport of transition metals3 Mon Introduction to the essential role of metals in photosynthesis:Mg in Chl, Mn in the Mn-cluster of PSII, non-heme iron in PSII, Fe in hemes and FeS-clusters in the Cytb6f-complex, copper in plastocyanin, FeS-centres in PSI, Ferredoxin, ...Tue Biogeochemical cycles of elements, in particular metalsWed *From physiology to biochemistry to spectroscopy and protein structure: copper and iron proteinsThu Nitrogen fixation and regulation of photosynthesis for nitrogen fixationFri * Bioinorganic chemistry of iron nutrition and iodine in marine algae4 Mon Literature seminars of the course participantsTue Ultra-Micronutrients Cr, NiWed Metal toxicity stress Metal Toxicity: basic principles (where do metals bind under toxic conditions, what are the physiological consequences, how can metal toxicity be analysed biochemically and biophysically)Thu Copper as an essential, but also highly toxic, biologically redox-active metal: from deficiency to toxicityFri * Arsenic toxicity and speciation in plants, incl. implications for human nutrition5 MonCadmium as a highly toxic but possibly also beneficial, biologically redox-inert metalTue- spare day -WedMetal resistance and detoxification Principles of metal detoxification in plants: Detoxification by formation of strong ligands, by sequestration by exclusion, by redox changes, etc.Thu * Metallothioneins: structure, biochemistry, physiological functionFri Biotechnological use of metal accumulation in plants (Phytoremediation, Phytomining)6 Mon Generally important scientific skills Literature searches and scientific writingTue How to give a good talk, how to prepare a good posterWed - spare day -Thu Final seminars of the course participants about their projects,partyFri cleaning upContent of practicals Basics, selected important methods, with emphasis on applications in bioinorganicchemistry and biophysics of plants, and special emphasis on methods for analysis in vivo, in situ and methods developed or improved in the Küpper group (e.g. FKM, GPS-fitting, QISH).The projects of this course originate from the current research of our department as described on our homepage (http://www.umbr.cas.cz/~kupper/AG_Kuepper_homepage.html). And as far as this is possible for a six-week project, they are independent subprojects, each supervised by a member of the department.

Literatura

Andresen E, Küpper H (2013) Cadmium Toxicity in Plants. In: Cadmium: From Toxicity to Essentiality, Chapter 13, Volume 11 of series "Metal Ions in Life Sciences". (Eds: Sigel A, Sigel H, Sigel RKO). Springer Science + Business Media B.V., Dordrecht; pp. 395-414. Leitenmaier B, Küpper H (2013) Compartmentation and complexation of metals in hyperaccumulator plants. Frontiers in Plant Science, doi: 10.3389/fpls.2013.00374. Reviews: Küpper H, Kroneck PMH (2005) Heavy metal uptake by plants and cyanobacteria. In: Metal Ions in Biological Systems, Band 44, Kapitel 5. (Hrsg.: Sigel A, Sigel H, Sigel RKO). Marcel Dekker, Inc., New York; pp. 97-142. Selected chapters from the following textbook: Crichton RC (2008) Biological Inorganic Chemistry - An Introduction. Elsevier B.V., Amsterdam, 369S.

Požadavky

Periodicita: předmět běží každý rokPředmět je určený pro studenty doktorského studia.

Garant

prof. Hendrik Küpper, Ph.D.

Vyučující

prof. Hendrik Küpper, Ph.D.prof. Hendrik Küpper, Ph.D.