Předmět Biochemistry 2 (UCH / 643)

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Materiál	Typ	Datum	Počet stažení

Obsah

Contents of lectures:1.Introductory to Biochemistry: The origin of life; Mechanisms of molecular interactions, Metabolism.2.Biomolecules: Amino acids; Peptides; Proteins; Carbohydrates; Lipids; Nucleic acids.3. Enzyme catalysis I: Principles of catalysts and enzyme activity.4. Enzyme catalysis II: Enzyme kinetics; Enzyme properties; Enzyme types.5. Gene expression and proteosynthesis: Mechanisms of DNA replication; Transcription/the synthesis of RNA; Gene regulation in Procaryotes; Gene regulation in Eucaryotes; Translation/the synthesis of proteins; Posttranslational modification.6. Biological membranes and membrane transport: Composition and structure; fluidity and assymmetry; transport across membranes; Methods in biomembrane research.7. Introductory metabolism and bioenergetics: Metabolism; Thermodynamics of energetic metabolism; Biological oxidation; Coupled reactions; Experimental approach.8. Energetic metabolism I: Carbohydrate metabolism; Compartmentation; Regulation.9. Energetic metabolism II: The Citric acid cycle; Electron transport; Oxidative phosphorylation.10. Energetic metabolism III: Lipid metabolism; Nitrogen utilization; Compartmentation.11. Energetic metabolism IV: Photosynthesis and photosynthetic machinery; Photorespiration; the C4 cycle.12. Biotransformation: Metabolism of xenobiotics; Mechanisms of biotransformation reactions; Enzymes in biotransformation, Compartmentation and physiological features.13. Integration and regulation of energetic metabolism: Compartmentation of metabolic pathways; Functinal relatioships between pathways; Regulatory actions; Metabolic check points; Experimental approaches in metabolism.Contents of laboratories:1.Isolation of nucleic acids from algae.2. Affinity chromatography of photosystem II..3. Separation of amino acids by ion exchange.4. Cleavage of gelatine by trypsin, Michaelis constant determination.5. Electrophoresis of proteins from thylacoid membranes.6. Determination of relative molecular weight of peroxidase by gel filtration.7. Kinetics of Hill`s reaction.8. Affinity chromatography of IgG.9. Isolation of lectins from the seesd of leguminous plants.10. Analysis of carbohydrates by anthrone agent.11. Isolation of horse radish peroxidase.12. Preparation of thylacoid membrane fractions on density gradient of succrose.13. Spectrophotometry determination of tyrosine and tryptophane in proteins.14. Quantitative analysis of glucose.15. Time course analysis of cleavage of gelatine by trypsin.16. Measurement of peroxidase activity: estimation of kinetic parameters.17. Isolation of mannan from yeast.18. Glycans of cellular glycoproteins: analysis by affiinity blotting.19. Imunoblotting of tick heamolymph with rabbit antibodies against lectin.20. Isolation of DNA from calf thymus.21. Isolation of DNA from liver of laboratory rat.

Literatura

A. L. Lehninger, D. L. Nelson, M. M. Cox: Principles of Biochemistry. Worth Publishers, New York, 1993. D. Voet, J. G. Voet: Biochemistry. Victoria Publishing Ltd. J. Stenesh: Biochemistry. Plenum Press,New York&London, 1998.

Garant

prof. RNDr. Libor Grubhoffer, CSc.

Vyučující

prof. RNDr. Libor Grubhoffer, CSc.prof. RNDr. Libor Grubhoffer, CSc.