Předmět Bioanalytics II - Laboratory medicine (C7943)
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Další informace
Cíl
main objectives of lecture:introduction into laboratory diagnostics;at the end of the course students should be able- to define relation between bioanalysis and illness diagnosis;- to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation;- to undestand principles of basic methodical approaches and common instrumental techniques.
Osnova
1. introduction, samples - preanalytical phase2. instrumentation - analysers; organisation, integration and miniaturisation of analyses3. quality in clinical analysis - check and control4. analytical methods - selection and optimisation5. analytical sets; analytical result - its presentation; selected instrumental methods6. colourness - physical phenomenon and its utilisation in bioanalysis; medical microbiology7. clinical immunoanalysis8. clinical aspects of protein analysis9. clinical aspects of DNA analysis10. case study - development of a method for clinical diagnostics; role of buffers in bioanalysis11. selected analytes 1 - inorganic12. selected analytes 2 - organic and macromolecules
Literatura
Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis, Mo.: Saunders/Elsevier, 2008. xx, 952 p. ISBN 978-0-7216-3865-2. infoClinical laboratory diagnostics :use and assessment of clinical laboratory results. Edited by Thomas Lothar. 1st ed. Frankfurt am Main: TH-Books, 1998. 1527 s. ISBN 3-9805215-4-0. info
Požadavky
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are pre-requisited.
Garant
doc. Mgr. Jan Havliš, Dr.
Vyučující
doc. Mgr. Jan Havliš, Dr.