Předmět Klinická biochemie (B80114)

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Materiál	Typ	Datum	Počet stažení

Literatura

Basic : G.Beckett et al. Lecture Notes: Clinical Biochemistry, 7th ed.Blackwell Publishing Ltd,2005J.Wallach. Interpretation of diagnostic tests. 7th ed. Lippincott Williams & Wikins,2000. A.Gaw et al. Clinical Biochemistry An illustrated colour text. Churchill Livingstone,1999.M.F.Laker. Clinical Biochemistry for Medical Students, Saunders W.B. Company Ltd. 1996. seminar lectures-see:http://www1.lf1.cuni.cz/ukb/lectures.htm Recommended: C.A.Burtis, E.R.Ashwood D.E.Bruns. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th ed.Elsevier Saunders W.B. Comp., Philadelphia 2006. F.Fischbach. A Manual of Laboratory & Diagnostic Tests. Lippincott, 2000. C.A.Burtis, E.R.Ashwood: Tietz Textbook of Clinical Chemistry. Saunders W.B. Comp., Philadelphia 1999.L.A.Kaplan, A.J.Pesce, S.C.Kazmierczak. Clinical Chemistry, Theory, Analysis, Correlation. 4th ed. Mosby, 2003. N.W.Tietz, R.B.Conn, E.L.Pruden. Applied Laboratory Medicine W.B.Saunders Comp., Philadelphia 1998. L.Thomas ed.Clinical laboratory diagnostics:use and assessment of clinical laboratory results.1.ed. TH-Books-Verl-Ges.,1998. P.D.Mayne et al.. Clinical Chemistry in Diagnosis and Treatment. Edward Arnold,1998. R.Montgomery, T.W.Conway, A.A.Spector. Biochemistry - A Case Oriented Approach. C.V. Mosby Comp., 1990. F.Fischbach A Manual of Laboratory & Diagnostic Tests.6thed. Lippincott Williams & Wilkins, 2000.

Požadavky

Questions Clinical biochemistry , 6th year, foreign students:Sodium, potassium, chlorideCalcium, phosphate, magnesiumProteins in plasma, albumin, total proteinEffective osmolality and its disorders,water homeostasisAcid base balance Urea, creatinine, glomerular filtration rate, urinary excretion of other metabolitesGlucose in blood and urine, ketone bodies, lactateTests for diabetes mellitus (oGTT,insulin,C-peptide,GAD Ab,HbA1,AGE and others)Urinalysis, examination of the urinary sedimentErythrocytes sedimentation rate (ESR), acute-phase reactantsAuto-antibodies,ImmunoglobulinsInvestigations of the impairment of the hepatocytes Investigations of the cholestasis Tests for synthetic functions of the liverAmylase, lipase, trypsin, elastaseInvestigation of the small intestine, function tests in gastroenterologyScreening tests in gastroenterologyTriglyceride, cholesterol, lipoproteinsIron, transferrin, ferritinTumor markersTrace elementsTests for bone remodeling and resorptionInvestigation of cerebrospinal fluidUrgent testsOxygen and lactate assessment ,monitoring of fluid and electrolyte balance in intensive care Body fluid ,its spaces , hyper and hypohydratationDifferential diagnosis (dif.dg.) of hyperbilirubinaemia and jaundiceDif.dg. of proteinuriaDif.dg. of hyper and hypoglycaemiaDif.dg. of hyper and hypouricaemiaDif.dg. of liver impairmentDif.dg. of pancreas impairmentInvestigation and dif.dg. of urolithiasisDif.dg. of acute heart diseasesInvestigation of testicular and ovarian involvementInvestigation of endothelium impairmentInvestigation and dif.dg. of the bone diseasesInvestigation of the porphyriasPrimary and secondary dyslipoproteinaemiasMetabolic acidosis and alkalosisRespiratory acidosis and alkalosisAssessment of renal functionDif.dg. of renal failureScreening of congenital defectsInborn errors of metabolism,basic indications for investigation,phenylketonuriaInvestigation of the lipid disordersInvestigation of thyroid disordersAssessment of the antioxidants capacityBiological influences on the laboratory results Preanalytical variables ? preparing of the patient, collection of the specimenPreanalytical variables ? transport and storagePoint of care testing, dry chemistryBasic principles of the laboratory techniques, photometry, chromatography, ELISATherapeutic drug monitoring, drug screeningCytogenetic analysis and its meaning in different clinical specialitiesThe use of basic methods in molecular cytogenetics Laboratory control of anticoagulants treatmentPreparation of samples for direct detection of infectious agensBasic principles of proper use of antibioticsAnemias

Garant

prof. MUDr. Tomáš Zima, DrSc., MBA