Předmět Lékařská mikrobiologie I (DA0104338)

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Materiál	Typ	Datum	Počet stažení

Sylabus

Syllabus - international students practical courseMedical Microbiology - 2nd year - summer semester1) Laboratory safety rules and equipment. Processing of clinical samples in microbiological analysis.Medical students will be familiarized with safety rules and with organization of education process at the Department of Medical Microbiology. Information including desinfection and sterilization will be presented and various microscopic will be demonstrated.2) Collection of clinical samples and transport.Demonstration of relevant collection procedures of significant clinical samples.3) Direct detection, part 1 (microscopy).Morphology of bacterial cell; microscopy preparation and fixation; various staining procedures.4) Direct detection, part 2 (cultivation, phenotypic identification, serotyping).The goal of the practical is cultivation of microorganisms using general, enriched and diagnostic culture media. Students are familiarized with fundamental terms as culture media and cultivation conditions. Students practise inoculation on culture media. The practical includes working with isolates, their identification with phenotypic method and reverse agglutination.5) Susceptibility to antibiotics.Students determine antibiotic susceptibility of various clinical bacterial isolates by using disc diffusion method. Minimum inhibitory concentration (MIC) using broth microdilution method and E-test will be demonstrated.6) Indirect serological methods.The most significant indirect (serological) methods (agglutination, precipitation, Enzyme-Linked ImmunoSorbent Assay - ELISA) are introduced. Serological results of the methods are presented and interpreted using knowledge of basic immunological mechanisms.7) Molecular biology methods.Overview of molecular biology methods applicable in medical microbiology laboratory.8) Staphylococci.Microbiological diagnosis of staphylococcal infection. Phenotypic identification using screening tests, ie. free and bound coagulase, catalase test.9) Streptococci.Differentiation of streptococci using their colony morphology, hemolytic properties and various tests as optochine test, PYR test, CAMP test.10) Bordetella, Haemophilus, Neisseria.Demonstration of supplemented enriched media which should be used for cultivation of the fastidious organisms. Instruction for deep nasopharyngeal swab to collect clinical material for detection of Bordetella pertussis or B. parapertussis.11) Enterobacteriaceae.Microbiological diagnosis of clinically important strains. Practical approach of reverse agglutination to confirm relevant serotypes.12) Pseudomonas and Nonfermenters.Microbiological diagnosis of significant clinical species. Detection of various pigment production and antibiotic resistance of clinical isolates.13) Listeriae and Corynebacteria.Cultivation of saprophytic and pathogenic corynebacteria using diagnostic culture media. Detection of strain toxigenity. Temperature dependent cultivation of Listeria monocytogenes.14) Credits.

Literatura

Bednář M., Fraňková V., Schindker J. akol., Lékařská mikrobiologie 1992, Marvil Praha

Garant

doc. MUDr. Pavel Dřevínek, Ph.D.