Předmět Lékařská mikrobiologie II (DA0105037)

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Materiál	Typ	Datum	Počet stažení

Sylabus

Medical Microbiology - 3rd year - winter semester1) Staphylococci - Identification of staphylococcal species using biochemical identification systems. Bound and free coagulase in differentiation of coagulase positive (e.g. S. aureus) and negative staphylococci.2) Streptococci - Colony morphology of clinically significant streptococci and their differentiation among β-hemolytic and group of Viridans streptococci using PYR, CAMP and optochine tests.3) Neisseria, Corynebacteria and Listeria - Colony morphology using enriched and selective media, C. diphtheriae - toxigenity test. Temperature dependent culture of L. monocytogenes.4) Enterobacteriaceae - Laboratory diagnosis of significant species and strains using biochemical tests and reverse agglutination method.5) Bordetella, Haemophilus and Pseudomonas - Fastidious nature of Haemophilus species and special enriched media. Specimen collection and diagnostic media to detect Bordetella species. Colony pigmentation and resistance of Pseudomonas aeruginosa strains.6) Anaerobic bacteria - Anaerobic culture conditions. Overview of clinical material collected from patients with suspected anaerobic infection. Identification of clinically significant anaerobic agents using biochemical tests. Spore-forming anaerobes of the Clostridium genus.7) Mycobacteria and Candida - Ziehl-Neelsen stain of acid-alcohol resistant mycobacteria; culture of medically significant mycobacterial strains. Yeasts and moulds - native prepration and culture of medically significant species.8) Interpretation of laboratory results - Overview of laboratory methods applicable in diverse clinical situations. Interpretation of microbiological and serological results.9) Parasitology - Microscopy and macroscopy in identification of clinically significant parasites. Summary of general parasitology.10) Molecular methods in clinical microbiology - Overview of molecular methods applicable in medical microbiology laboratory - benefit and limitation.11) Specimen collection and transport of clinical material - Applicable methods to collect and transport clinically significant clinical specimens. Gram staining procedure.12) Processing of respiratory and urogenital specimens to detect infectious agents.13) Processing of gastrointestinal specimens - Diagnostics of bacterial (Salmonella, Shigella, Campylobacter) and other agents. Transmission of the agent and relevant therapeutical properties.Review - overview of relevant clinical material and diagnostic procedures.14) Consultation, credit - Completed credit test and absolute presence is required to receive the credit.

Literatura

Murray, P. Medical microbiology : Mosby, 2009 (B), (avaible as ebook)+Mims, C. A.: Medical microbiology : Mosby, 2013

Garant

doc. MUDr. Pavel Dřevínek, Ph.D.