Předmět Biochemie I (FD10016)

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Materiál	Typ	Datum	Počet stažení

Sylabus

LecturesNomenclature of Inorganic Compounds. Atomic Structure. Electron Configuration and the Aufbau Principle. (Stoklasová 2)Elemens and their Compounds. Biogenic Elements (Stoklasová 1)Periodic System of Elements. Trends in the Periodic Table. (Stoklasová 2)Chemical Bonding - Covalent, Ionic and Metallic Bonds. Intermolecular Forces - Dipole-Dipole Forces, Hydrogen Bond, the London Forces. (Stoklasová 2)Classification of Chemical Reactions. Chemical Thermodynamics - the Laws of Thermodynamics, Enthalpy, Entropy, Free Energy. Spontaneity of Chemical Change. Chemical Equilibrium. Equilibrium Constant. Le Chatelier´s Principle. Chemical Kinetics. Reaction Rates and Factors that Influence them. Activation Energy and the Activated Complex. Catalysts and Mechanism of their Effect. (Stoklasová 2)Solutions and their Properties. Solubility, Concentration of Solutions. Solutions of Electrolytes, Ionization Constant. Activity (effective concentration). Acids and Bases. The Dissociation of Water. The pH Scale. Salts, Hydrolysis of Salts, Solubility Product. (Tomšík 3)Buffers, Characterization, pH, Capacity. Buffers of the Blood. (Tomšík 1)Oxidation-Reduction Processes. Hydrogen and Oxygen in these Processes. Standard Reduction Potentials. (Tomšík 1)Osmosis. Osmotic Pressure. Colloidal-Osmotic Pressure. Colloidal State - Types of Colloids, Properties. Surface Phenomena. (Cerman 2)Scope of Organic Chemistry. Formulas, Naming and Classification of Organic Compounds. (Mrkvicová 1)Hydrocarbons and their Derivatives. Alkanes, Alkenes, Alkynes, Cycloalkanes, Aromatic Hydrocarbones; Alkyl- and Aryl Halides, Amino-, Nitro-derivatives of Hydrocarbons; Thiols, Sulphides, Disulphides, Sulphonic Acids; Alcohols, Phenols; Aldehydes, Ketones; Carboxylic Acids and their Derivatives. Urea and its Derivatives. Environmental Pollution. (Mrkvicová 5)Heterocyclic Compounds with one or more Heteroatoms, their Importance in Biochemisty. (Mrkvicová 1)Amino Acids and their Physical and Chemical Properties. Peptides. Proteins - Amino Acid Composition, Conformation of Proteins - Types of Bonds. Classification of Proteins. (Cerman 3)Enzymes - Characterization, Mechanism of their Action. Vitamins as Cofactors of Enzymes. Kinetics of Enzymatic Reactions, the Michaelis Constant. Environmental Effects Influencing Enzyme Activity. Inhibition of Enzyme Activity. Nomenclature and Classification of Enzymes. Enzymes in Medicine. (Cerman 3)Overview of Intermediary Metabolism. Biological Oxidation and Bioenergetics. The Citric Acid Cycle and its Regulation. (Řezáčová 2)Carbohydrates. Monosaccharides - Classificaton, Configuration. Optical Activity, Anomers, Epimers. Reactions of Monosaccharides. Glycosidic linkage. Reducing and non-reducing Disaccharides. Polysaccharides and Glycosaminoglycans - Composition, Properties. Digestion.  Metabolism of Glucose, Fructose, Galactose and Polysaccharides. (Řezáčová 5)Lipids - Classification and Properties. Waxes. Digestion. Metabolism of Triacylglycerols, Phospholipids, Fatty Acids. Cholesterol and its metabolism. (Tomšík 4)Laboratory classes & seminarsLecturers Prof. Martina Řezáčová M.D., Ph.D.Prof. Jiří Stulík, M.D., Ph.D.Assoc. Prof. Jaroslav Cerman, M.D., Ph.D.Assoc. Prof. Alena Stoklasová, M.D., Ph.D.Miloš Hroch, Ing., Ph.D.Renata Köhlerová, MS, Ph.D.Alena Mrkvicová, MS, Ph.D.h.D.Dr. Mohamed Niang, Ph.D.Martina Seifrtová, MS, Ph.D.Pavel Tomšík, M.D., Ph.D.SeminarsNomenclature of Inorganic Compounds. The Periodic Law. Periodic system. Chemical Bond. Interactions among Molecules. Basic Calculations in Chemistry. Chemical Reactions.  Oxidation-Reduction Reactions. Test.Inorganic Chemistry. Themodynamics and Kinetics of Chemical Reactions.Electrolytes, Electrolytic Dissociation. The Bronstedt-Lowry Concept. The pH of Acids and Bases. Buffers. Calculations.Test.Hydrocarbons, Alcohols, Phenols, Quinones, Nitrogen- and Halogen-derivatives, Sulphur-containing Compounds, their Rections. Heterocycles. Terpenes. Importance in Biochemistry. Test.Aldehydes, Ketones, Organic Acids, their Reactions. Urea and its Derivatives. Importance in Biochemistry. Test.Heterocycles. Vitamins. Synthetic Materials, Importance in Dentistry. Test.Colloidal Solution, Osmotic Pressure. Importance in Biochemistry. Amino Acids, Peptides, Proteins. Nucleic Acids.Enzymes, Structure, Mechanism of their Action. The Michaelis Constant. Factors Affecting the Rate of Enzyme Catalyzed Reactions. Isoenzymes.Disinfectans, Anestetics. Credit test.Biological Oxidation. The Respiratory Chain. Metabolism of Oxygen. Test.Citric Acid Cycle. Carbohydrates - Classification, Structure, Properties. Artificial Sweeteners.Metabolism of Carbohydrates - Glucose, Fructose, Galactose, Uronic, Acid. Microorganisms and Metabolism of Carbohydrates.Test.Glycosaminoglycans, Glycoproteins. Proteoglycans. Lipids - Classification, Structure, Properties.Laboratory classesLaboratory Practice and Safety Rules. Chemical Glass. Preparation of Pure Inorganic Substances.Spectrophotometry. Determination of ferrous Ions Concentration.Potenciometry. Weak Acid - Titration Curve.The pH Measurement. Determination of Capacity of Acetate Buffer.Factors Influencing Laboratory Results. Accuracy of Measurement.Properties of Enzymes - the Influence of pH and Temperature. Substrate Specificity. Activity of Salivary Amylase.Notes:Attendance at laboratory classes and seminars is COMPULSORY and a register is kept. Substitution of written tests must be consulted with Asssoc. prof. Stoklasová in case of your unavoidable absence (e. g. illness). Before starting laboratory class, do not forget laboratory coat and change your shoes (with rubber sole).  Calculator is necessary. Laboratory manual is available in MOODLE.

Literatura

Compulsory literatureR. Y. Ouellette Introduction to General, Organic and Biological Chemistry. 3rd ed., Mac Millan Publ.Company, New York, 1992. ISBN 0-02-390245-0 (or the latest edition available)R. K. Murray et al. Harper's Biochemistry. 24th ed., Appleton and Lange, Stamford, USA, 1996.ISBN 0-8385-3612-3. (or the latest edition available) Recommend literatureZ. Durackova et al. Medical Chemistry. Asklepios, Bratislava, 1993. ISBN 80-7167-003-0P. C. Champe and R.A.Harvey Biochemistry. Lippincott Co, 1987. ISBN 0-397-50801-8 (or the latest edition available)J.W. Baynes et M. H. Dominiczak Medical Biochemistry. 4th ed., Elsevier 2014. ISBN 978-1-4557-4580-7

Požadavky

Credit:a) Attendance at practical courses and seminars b) Complete protocols c) Result of all examinations: at least 3.00 (written tests and oral examinations - the credit test is not included). Written tests are obligatory and missing tests must be substituted. d) Result of the credit test: at least 3.00  

Garant

doc. MUDr. Alena Stoklasová, CSc.