Předmět Biologie II (FG10012)

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Materiál	Typ	Datum	Počet stažení

Sylabus

LecturersProf. Dr. Miroslav Cervinka, M.D., Ph.D.Prof. Dr. Emil Rudolf, Ph.D. October 7The mitochondrion, production of ATP, mitochondrial DNA.The cytoskeleton (microtubules, microfilaments, intermediate filaments), cell motility, movement of organels, cellular motors.         October 14Cell growth and cell division, cell cycle, mitosis.Cell death, apoptosis.        October 21Gene, genetic code.Mutations, reparation of damaged DNA, screenings for mutagens.Chromosomes, human karyotype. Structural and numerical aberrations of autosomes.               November 4Sexual reproduction, meiosis, abnormalities of gonosomesX-linked genes, X chromosome inactivation.               November 11Genetic manipulations - basic methods and principles.Recombination of DNA, PCR, cDNA.               November 18DNA sequencing, genomics.                November 25DNA diagnostics.Gene therapy.              December 2Genetics of multicellular organisms, Mendel’s law, Morgan’s law, pedigree analysis.               December 9Twins in genetic research, cytogenetics, prenatal diagnosis.Population genetics.               December 16Metabolic disorders.Clinical genetic.   Practical courses  LecturersProf. Dr. Emil Rudolf, Ph.D.Dr. Vera Kralova, Ph.D. 7., 9. 10.              1.            The cell cycle and cell division1.1          Mitosis in permanent mounts of plant and animal cells1.2          Mitosis in cells of cell cultures1.3          Determination of the mitotic index and particular phases of mitosis                              14., 16. 10.         2. A        Karyotyping using human cells (1st subgroup)2. B        The summer term DNA samples- electrophoresis (2nd subgroup)                              21., 23. 10.         3. A        The summer term DNA samples- electrophoresis (1st subgroup)3. B        Karyotyping using human cells (2nd  subgroup)                              30. 10.  4.            Eukaryotic chromosomes and meiosis4.1          Meiosis in cells of locust 4., 6. 11.              5.            Cytogenetic tests and their use in clinical genetics5.1          Chromosome identification and classification (banding, SCE, FISH and other techniques)                                                             11., 13. 11.         6.            Sex chromatin6.1          Sex chromatin in leukocyte smear6.2          Evaluation of the prepared karyotypes                              18., 20. 11.         7.            Mendelian genetics-pedigrees (workshop I)7.1          Pedigree analysis, symbols and rules applied                              25., 27.11.          8.            Molecular biology techniques in human genetics               Biological typing in man (workshop II)8.1          DNA diagnostics                              2., 4. 12.              9.            Tumor biology I.9.1          Normal and tumor cell in vitro and in vivo9.2          Karyotypes of normal and tumor cells                              9., 11. 12.           10.         Tumor biology II. (seminar)10.1       Cell malignant transformation - mutagenesis and carcinogenesis                              16., 18. 12.         11.         Population genetics (seminar)11.1       Introductory concepts - Hardy-Weinberg law                              6., 8.1. 2015       12.         Advances in molecular biology in 201412.1       Credit test12.2       Students presentation I                              13., 15. 1.13.         CREDIT13.1       Students presentation II                                 

Literatura

Compulsory literatureB. Alberts et al. Essential Cell Biology. An introduction to the molecular biology of the cell. Garland Publishing, Inc. New York & London, 1998. ISBN 0-8153-2971-7M. Connor et al. Essential Medical Genetics. 5th ed., Blackwell Science Ltd., 1997. ISBN 0-632-04158-7Nussbaum, McInnes, Willard Thompson and Thompson Genetics in Medicine. 6th ed. revides reprint, Saunders, 2004. ISBN 0-7216-0244-4 Lectures and recommended literaturHandouts and other materials provided by the Department, homepage of Department of Medical Biology and Genetics.

Požadavky

Requirements for credit a) Attendance + active participation Attendance at practical classes is mandatory. Each student is allowed one absence (medically excused) from one practical class. Practicals begin at 11.10 a.m. and students are required to be in their seat. If the student is not present at that time, he will be marked absent. Students must be prepared for the class. b) Report, karyotype, presentation, manual Studentsareobligedtohandintheirownkaryotypealongwithareferencephotograph.Thedeadlineis November 22th 2013. Students are also obliged to preparean abstract of their oral presentation and submit this abstract via email for checkout.The topics will be specified at the beginning of the semester. The deadline is December 6th 2013 . In case of the inappropriate presentation topic, we reserve the right to reject it and ask for the refinement or topic change. The full text version of presented topics should be submitted by e-mail before December 20th 2012. Manualpresentedduringcreditsessionmusthaveanappropriateexternalform;i.e.beclean,intactasithasstatusofofficialdocument. c) Tests There is one final test which must be passed. Passing grade is 75 %. In case of the test failure, the student has two more chances to pass written make-up tests. d) Credit The credit in this term consists of: Final test with minimal scoring grade of 75 % Completedmanualincludingdrawingsandanswerstoquestions (in case of any manual damage or loss, the student has to acquire a new one from the department against financial compensation). Preparedkaryotype Successfully presented topic (evaluation of presentation will include the topic itself as well as the quality of presentation) Appointmentsforwintercreditarescheduledtowinter examination period (January 20st 2014 - February 16th 2014), summer examination period (June 2rd 2014 - June 29 th 2014) andduring September (1. - 26. 9. 2014),each week on Thursdays from 2 to 3pm only. Generally, in case of failure in credit (final test), the next possible chance is after one week!!!! The students who seek the exemption from practical classes in medical biology and genetics have to submit their request before the start of practical classes. Their request must be accompanied by a detailed syllabus of biology course they had completed which will be evaluated by the head of the department of medical biology and genetics. e) Examination Before signing for the final examination each student must have two credits, one credit in the summer term of the first year and the second one in the winter term of the second year. Cheating during any form of biology exam (credit test and oral examination) including preparatory period is not tolerated! Use of any electronic devices (cell phones, tablets, notebooks, phablets etc.), unauthorized materials or communication with other students in these instances is strictly prohibited! After selecting final exam questions it is not allowed to leave the room and when necessary an escort will be provided. In case of failure to comply with these rules, student will be classified "failed".

Garant

prof. MUDr. RNDr. Miroslav Červinka, CSc.