Předmět Biochemie II (FG10014)

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Materiál	Typ	Datum	Počet stažení

Sylabus

LecturesLipids and Steroids. Classification, Structure, Properties, Chemical Reactions. (Stoklasová 1) Metabolism of Lipids and Steroids - Digestion, Resorption and Transport.  Biosynthesis and Degradation of Saturated and Unsaturated Fatty Acids. Ketogenesis. Eicosanoids. Metabolism of Acylglycerols and Sphingolipids. Metabolism of Cholesterol. Biosynthesis of Bile Acids and Steroid Hormones. (Stoklasová 4) Metabolism of Proteins and Amino Acids -Digestion, Resorption and Transport. Transamination, Oxidative Deamination and Decarboxylation of Amino Acids. Catabolism of the Carbon Skeleton of Amino Acids. Ammonia Formation and its Removal. Biogenic Amines. (Řezáčová 4) Metabolism of Porphyrins and Bile Pigments. Biosynthesis of Haeme and Haemoglobin and their Catabolism. Jaundice. (Řezáčová 2) Components of the Blood. Water and Ions Metabolism. Acid-Base Balance. TLungs and Kidneys in Acid-Base Balance. Disorders of Acid-Base Balance.  (Stoklasová 3) Metabolism of Erythrocytes. Haemostasis and Blood Clotting. (Stoklasová 2) Metabolism of Kidneys. (Řezáčová 1) Metabolism of Muscles. Contractile and Structural Proteins. Mechanism of Muscle Contraction and its Energy Supply. (Tomšík 2) Metabolism of Connective Tissue. Connective Tissue Proteins, Proteoglycans. (Tomšík 2)Metabolism of Bones and Teeth. Process of Mineralization. (Stoklasova 1) Digestive Tract and Liver. Importance in Metabolism. (Řezáčová 2) Metabolism of Nerve System (Řezáčová 1) Metabolism of Adipose Tissue. (Řezáčová 1) Introduction to Xenobiochemistry. Two Phases of Biotransformation. (Tomšík 2) Lipoproteins and their Metabolism. (Stoklasová 1)Extracellular and Intracellular Communication. Second Messengers, Protein Kinases. (Cerman 2)Hormones and their Action. Synthesis, Degradation, Effects: Hormones of Hypothalamus, Pituitary Gland, Thyroideal Gland, Parathyroideal Gland, GIT, Pancreas and Adrenal Medulla. (Cerman 4)         The Comparison of the Metabolic Pathways in Liver, Muscles, Adipose Tiissue and CNS. (Stoklasová 2) Introduction to Immunochemistry. (Stulík 3)   Practical courses & seminarsLecturersAssoc. Prof. Jaroslav Cerman, M.D., Ph.D.Assoc. Prof. Martin Beránek, Ph.D.Prof. Martina Řezáčová, M.D., Ph.D.Assoc. Prof. Alena Stoklasová, M.D., Ph.D.Dr. Renata Kohlerová, Ph.D.Dr. Alena Mrkvicová, Ph.D.Dr. Darina Muthná, Ph.D.Dr. Mohamed Niang, Ph.D.Dr. Pavel Tomšík, Ph.D.Dr. Alžběta FilipováIng. Miloš Hroch, Ph.D.Dr. Eva PeterováDr. Lucie Podmolíková  SeminarsMetabolism of Lipids - Digestion, Absorption, Transport. Metabolism of Fatty Acids, Triacylglycerol, Phospho-, Sphingo- and Glycolipids.  Test.Metabolism of Steroids - Classification, Structure, Properties. Metabolism of Cholesterol, Bile Acids and Steroid Hormones. Ketone Bodies. Test.Metabolism of Amino Acids and Proteins - Digestion, Absorption, Catabolism of Amino Acids. Metabolism of Ammonia. Biosynthesis of Urea. Test.Metabolism of Porphyrins and Bile Pigments. The Blood, Blood Clotting. Importance of Eicosanoids. Presentations.Acid-Base Balance.Minerals and Water in the Body and their Regulation. Metabolism of Iron and Copper. Test.Metabolism of Connective and Contractile Tissues. Presentations.Metabolism of Bones and Teeth and their Hormonal Regulation. Presentations.Metabolism of Lipoproteins, Hyperlipoproteinaemias. Metabolism of Adipose Tissue. Presentations.Metabolism of Xenobiotics. Presentations.Extracellular and Intracellular Communication. Receptors. Metabolism of Nerve System. Biochemistry of Vision. Presentations.Structure and Properties of Hormones of Hypothalamus, Pituitary Gland, Thyroideal Gland and Adrenal Medulla. Regulatory Peptides in GIT.          Relationship between Metabolism of Carbohydrates, Lipids and Proteins in some Tissues and their Regulation. Hormones of Pancreas and their Importance. Test.Practical coursesIntorduction to the Chemical Methods in Clinical LaboratoryGlucose Concentration in the Serum. Catalytic Concentration of Choline Esterase in the Serum.Thin-Layer Chromatography (TLC) of the Lipid Components of the Serum. Concentrations of Total and HDL-Cholesterol. Triacylglycerols in the Serum.Protein Concentration in the Serum. Separation of the Serum Proteins by Electrophoresis.Concentrations of Urea and Uric Acid in the Serum. Concentration of Creatinine in the Serum and Urine.Concentrations of Calcium and Phosphates in the Serum. Concentration of Chlorides in the Urine. Presentation.Iron Concentration in the Serum. Total and Conjugated Bilirubins in the Serum. Concentration of Sodium and Potassium in the Serum.Liver Diseases. Catalytic Concentrations of Alanine Aminotransferase, Alkaline Phosphatase and gamma-Glutamyl Transferase in the Serum. Presentation.Heart Diseases. Catalytic Concentrations of Creatine Kinase, Aspartate Aminotransferase and Lactate Dehydrogenase in the Serum. Presentation.Qualitative Analysis of the Urine. Proteins, Glucose, Blood, Acetone and Ehrlich-positive Substances. Presentation.Notes: Attendance at practicals classes and seminars is COMPULSORY and a register is kept. For unavoidable absences (e. g. illness) a written note must be given to doc. Stoklasová immediately on your return. 

Literatura

Compulsory literatureR. Y. Ouellette Introduction to General, Organic and Biological Chemistry. 3rd ed., Mac Millan Publ.Company, New York, 1992. ISBN 0-02-390245-0 (or the latest edition available)R. K. Murray et al. Harper's Biochemistry. 24th ed., Appleton and Lange, Stamford, USA, 1996.ISBN 0-8385-3612-3. (or the latest edition available)J.W. Baynes et M. H. Dominiczak Medical Biochemistry. 4th ed., Elsevier 2014. ISBN 978-1-4557-4580-7 Recommend literatureP. C. Champe and R.A.Harvey Biochemistry. Lippincott Co, 1987. ISBN 0-397-50801-8 (or the latest edition available) 

Požadavky

Credit:a) Attendance at practical classes and seminars (3 absences will be tolerated)b) Complete protocols.c) At least pass average (3.00) in written tests and oral examinations. Substitution of test you miss is necessary to discuss with doc. A. Stoklasová.d) Power point presentation - a given topic. Examination:The examination consists of two parts - multiple choice questionnaire test and oral examination - and it is conditioned with having both credits. Written test:  at least 11 points are necessary for oral part of examination. The students who fail to satisfy the requirements of the examination are re-examined orally (two possibilities). 

Garant

doc. MUDr. Alena Stoklasová, CSc.