Předmět Mikrobiologie I (FG10017)

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Materiál	Typ	Datum	Počet stažení

Sylabus

LecturersAssoc. Prof. Vladimir Buchta, M.Sc., Ph.D.Zuzana Cermakova, D.V.M., Ph.D.Vlasta Stepanova, M.D., Ph.D.No. Theme Lecturers Duration       Introduction to medical microbiology. (Assoc.Prof. Žemličková)Practical courses & seminarsLecturersAssoc. Prof. Vladimir Buchta, M.Sc., Ph.D.Zuzana Cermakova, D.V.M., Ph.D.Vlasta Stepanova, M.D., Ph.D.Practical coursesMicrobiology Safety, devices, equipment, workflow in microbiology lab. Students are introduced into safety rules in microbiological laboratory and teaching organizations. Attention is paid to the issue of disinfection and sterilization. Hand hygiene.Preanalytical phase. Collection, transport, and maintenance of biological materials. Demonstration of correct procedures of collection and transport of clinically significant samples.Basic principles of microbiological diagnostics. Direct tests Part 1 (microscopy). Description of bacterial cell, staining, microscopy techniqueBasic principles of microbiological diagnostics. Direct tests Part 2 (cultivation, biochemistry, serology). Cultivation, culture media, length of cultivation, other factors. MALDI-TOF MS.Establishment of antibiotic susceptibility Antibiotic susceptibility methods, qualitative/ quantitative examination antibiotic susceptibility; break-point, interpretation of test results.Basic principles of microbiological diagnostics. Indirect tests. Microbiological methods in virology. Serological methods - principles (agglutination, precipitation, complement-fixation, ELISA). Virology - direct examination.Basic principles of microbiological diagnostics. Microbiological methods in mycology and parasitology. Molecular-biological methods. Microscopy, cultivation. Principle of PCR, the pros and cons. Indication of the methods in clinical practice.Credit test.Postanalytical phase. Interpretation of microbiological methods, human microbiota; primary and opportunistic pathogens. Credit.Notes: Actual informations about microbiology lectures and practical classes you can find on the board on the first floor of the department of microbiology (the building No. 17, Faculty Hospital).  

Literatura

Compulsory literatureW.A. Strohl, H. Rouse, B.D. Fischer Microbiology, Lippincott Williams a Wilkins, 2001,ISBN 0-397-51568-5W. Levinson, E. Jawetz Medical Microbiology and Immunology. 4th ed.,Appleton and Lange, Stamford, USA, 1996. ISBN 0-8385-6225-6 (and newer editions)Recommend literatureG. F. Brooks et al. Medical Microbiology. 3th ed., Lange, 2004. ISBN 0-07-141207-7C. A. Mims et.al. Medical Microbiology. Mosby, 1993. ISBN 0-397-44631-4 (and newer editions) 

Požadavky

CreditCredit will be given to students who:fulfill the attendance requirements (1 legitimate absence per term)have their written protocols in orderhave an average mark of tests up to 3,0

Garant

doc. MUDr. Helena Žemličková, Ph.D.