Předmět Kmenové buňky a regenerativní medicína (FG50019)

Na serveru studentino.cz naleznete nejrůznější studijní materiály: zápisky z přednášek nebo cvičení, vzorové testy, seminární práce, domácí úkoly a další z předmětu FG50019 - Kmenové buňky a regenerativní medicína, Lékařská fakulta Hradec Králové, Univerzita Karlova v Praze (UK).

Materiál	Typ	Datum	Počet stažení

Sylabus

LecturesLecturers  Prof. Jaroslav Mokry, M.D., Ph.D.  Prof. Stanislav Filip, M.D., Ph.D.  Assoc. Prof. Pavel Zak, M.D., Ph.D.  Tomas Soukup, M.D. 1    Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research. (Mokry, 3) 2    Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors. (Soukup, 3)  3    Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Muscle-derived stem cells; muscle regeneration. (Mokry, 3)  4    Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages Orthopaedic applications. (Soukup, 3) 5    Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury. (Mokry, 3)  6    Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation. (Mokry, 3)  Practical courses and seminarsLecturers  Tomas Soukup, M.D.  Jakub Suchanek, M.D., Ph.D. Practical courses1 Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.2 Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.3 Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation.4 Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.5 Visit of tissue culture laboratory. Cell viability. Preparation of cells for flow cytometric analysis. 

Literatura

Recommended  literature1. Alberts et al Molecular Biology of the Cell, Garland, ISBN 0815340729 2. Sell, S. Stem Cell Handbook, Humana Press, 2004,4th Editoion,2002, E-ISBN 1-59259-411-5 3. Turksen, K. Adult Stem Cells, Humana Press, 2004, E-ISBN 1-59259-732-7 4. Clark et al. The Molecular and Cellular Biology of Wound Repair, Plenum Press, 1996, ISBN 030645159X 5. Doyle et al. Mammalian Cell Culture (Essential Techniques), John Wiley and Sons, 1997, ISBN 0-471-97057-3 6. Bronzino et al. The Biomedical Engineering Handbook, CRC Press, 1999 7. Ratner et al. Biomaterial Science, Academic Press, 1997, ISBN 012824610 8. Patrick et al. Frontiers in Tissue Engineering, Pergamon, 1998, ISBN 0080426891 9. Lanza et al. Principles of Tissue Engineering, Academic Press, 2000, ISBN 012436630910. Frank H. Stephenson Calculations for Molecular Biology and Biotechnology, Academic Press, Elsevier Science 2003, ISBN 0-12-665751-3 

Požadavky

Presence in lectures and laboratory classes. Absences may be substituted only by writing an assay on the topic you missed. Elaboration of protocol for cell viability.

Garant

prof. MUDr. Jaroslav Mokrý, Ph.D.MUDr. Tomáš Soukup, Ph.D.