Předmět Mikrobiologie I (EA0104510)

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Sylabus

Microbiology exercises for general medicine I. (summer semester) 1) Introduction - safety, classification of microbes, characterization of main groups of microbes2) Dizinfection and sterilization - fyzical and chemical methods  and agents, indication and quality control, laboratory equipment and its application3) Cultivation I. - specimens collection, processing and storage, culture media characteristics4) Cultivation II. - seeding of culture media, characteristics of  microbial growth5) Microscopy I. - Gram stain, bacterial morphology6) Microscopy II. - Acid-fast stain, negative stain, slides of clinical specimens, host cells identification7) Cultivation III. - Biochemical and additional tests8) Cultivation IV. - Antibiotic susceptibility testing9) Immunological methods I.- terms definition, polyclonal and monoclonal antibodies charteristics, production and application,10)  Immunological methods II. - Principles of direct and indirect immunological methods11) Immunological methods III. - Main assays, its characteristics, application and limits in diagnostics12) Identification of medically important microbes - main groups of  microbes in cultures and microscopy slides