Předmět Methods of functional genomics (MB140P86)
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Další informace
Sylabus
2011 lecturer team will comprise (without titles):Vladimir Benes [benes@embl.de] - genomics http://www.embl.de/services/core_facilities/genecore/members/index.php?s_personId=147Jeroen Krijgveld [jeroen.krijgsveld@embl.de] - proteomicshttp://www.embl.de/research/units/genome_biology/krijgsveld/index.htmlJonathon Blake [blake@embl.de] - bioinformaticshttp://www.embl.de/services/core_facilities/genecore/index.htmlAndy Riddell [riddell@embl.de] - flow cytometryhttp://fccfweb.embl-heidelberg.de/fccfweb/index.php?option=com_contact&task=view&contact_id=1&Itemid=93Beate Neumann [bneumann@embl.de] - systematic siRNA approacheshttp://www.embl.de/almf/almf_services/contact/neumann/Tobias Rausch [rausch@embl.de] - bioinformaticshttp://www.embl.de/~rausch/Hüseyin Besir [besir@embl.de] - protein expression and purificationhttp://www.embl.de/services/core_facilities/pepcore/members/index.php?s_personId=CP-60003254The covered topics will include:Genomics:Microarrays - overview of technologies & applications;Massively parallel sequencing - overview of technologies & applications; comparison with microarraysqPCR - principles, applications, dataProteomics:Mass spectrometry: what?s the purpose?MS instrumentationPeptide fingerprinting: protein identification by MSPeptide sequencing by MSIdentification of post-translational modificationsLC-MSMS and large-scale proteomics, incl. examplesStable-isotope labeling and quantitative proteomicsAnalysis of intact proteinsBioinformatics:Introduction: Biologists and Bioinformatics>What bioinformatics is>What bioinformatics can do>What it can?t do>Current Challenges for bioinformatics >Data sizes, Read lengthsApplication 1: Microarrays>Analysis>Normalization>Design>Data Repositories and MiningApplication 2: Next Generation Sequencing>The range of applications>Data analysis by application>What can go wrong and biases with countsFlow cytometry:The principles of flow cytometryTechnology overviewAssays, applicationssiRNAThe principles of siRNAsTechnology overviewApplications, examples, including high-throughput screeningsProtein expression and purificationIntroduction to various protein expression systems and technologiesApplications, examples, troubleshooting
Literatura
Lectures reflect development in technologies and our current knowledge and change every year. Therefore teachers will prepare a new batch of recommended literature for each course. The recommended literature will be available for students enrolled to the course on http://web.natur.cuni.cz/~pospisek/vyuka/vyuka.htm in advance.
Požadavky
Final exam will be performed preferentially as a computer test but might comprise an oral exam or combination of both. The exam scope is bounded by the syllabus and information provided during the course.
Garant
RNDr. Vladimír Beneš, CSc.