Předmět Histology - a practical course (MB150C27E)
Na serveru studentino.cz naleznete nejrůznější studijní materiály: zápisky z přednášek nebo cvičení, vzorové testy, seminární práce, domácí úkoly a další z předmětu MB150C27E - Histology - a practical course, Přírodovědecká fakulta, Univerzita Karlova v Praze (UK).
Top 10 materiálů tohoto předmětu
Materiály tohoto předmětu
| Materiál | Typ | Datum | Počet stažení |
|---|
Další informace
Sylabus
1. Preparation of histological specimens: the procurement of tissues and organs (mouse), fixation, drainage using ascending series of alcohol, embedding in paraffin, cutting on a microtome, staining with hematoxylin / eosin, closing in Canadian balsam. 2. Preparation of smearing specimens of blood and bone marrow: methanol fixation, Giemsa 3. Preparation of stamping specimens of selected organs - spleen, thymus, lymph nodes, testes, cerebellum fixation with methanol, Giemsa 4. Preparation of fluorescently labeled specimens (detection of plasma cells in the spleen, characterization of the developmental stages of spermatogenesis in the testis) 5. Preparation of immunofluorescent slides for fluorescence microscopy (fixation, permeabilization blocking, staining with primary and secondary antibodies, DAPI, embeding in Mowiol)
Literatura
For this practical is not required any special literature, protocols are provided. At the beginning of the practical exercises all issues are thoroughly discussed and all protocoarer are available.
Požadavky
For getting the credits the 100% attendance is obligatory. Students have to prepare set of required specimens and their capture their images using a microscope equipped with a color camera with high resolution - both in the fluorescence mode and in transmitted light.
Garant
doc. RNDr. Jan Černý, Ph.D.