Předmět Epigenetics (MB150P85)

Na serveru studentino.cz naleznete nejrůznější studijní materiály: zápisky z přednášek nebo cvičení, vzorové testy, seminární práce, domácí úkoly a další z předmětu MB150P85 - Epigenetics, Přírodovědecká fakulta, Univerzita Karlova v Praze (UK).

Materiál	Typ	Datum	Počet stažení

Sylabus

an advanced course for M.Sc. and PhD students (the course is in English) signing up for the course is HIGHLY RECOMMENDED only after completing bachelor studies, ideally in the second year of the masters program. contacts: mail: svobodap@img.cas.cz, phone: 241063147COURSE PROGRAM:IntroductionOverview of the course, basic concepts of epigenetic marks, diversity of epigenetic mechanisms and effectsHistone modification IConcept of chromatin structure. Heterochromatin and euchromatin. Core histones, linker histones, replacement histones, protamines. Methods for studying chromatin.Histone modification IIHistone modifications, polycomb proteins, acetylation, fosforylation and histone methylations, effects on gene expression.DNA methylation IMolecular basis of DNA methylation. CpG and non-CpG methylation. Adenosin methylation. Metods for studying DNA methylation. Bisulfite sequencing.DNA methylation IIEffects of DNA methylation on gene expression, Methyl-binding proteins and mechanisms of inhibition of gene expression, distribution of DNA methylation within genes and mammalian genomes.ImprintingConcept of imprinting, mammalian imprinting. Molecular mechanisms of imprinting. Role of imprinting, Battle of the sexes. X-inactivationPrinciples and different strategies for dosage compensation. Control of X-inactivation in mammals.Epigenetic reprogramming in the mammalian life-cycleIntegration of epigenetic modification in the mammalian life cycle. Reprogramming of gene expression during development, artificial reprogramming - the traditional view.Epigenetic mechanisms found in other model systems (plants, yeasts, invertebrates ...)Selected epigenetic mechanisms controlling genome integrity and gene expressionRNA silencing I - molecular machines for RNA silencingA historical introduction into RNA silencing. Post-transcriptional effects. Roles and effects of dsRNA. Proteins and complexes in RNA silencing. RNA silencing II - RNAi technologyExperimental and therapeutic use. Design of RNAi experimentsRNA silencing III - roles of RNA silencing pathwaysmiRNA pathway, chromatin connection. Integrated view of regulation of gene expressionEstablishment and maintenance of pluripotency in ES cells and embryosStudents are expected to actively participate during the course.

Literatura

Studenti dostávají ke každé přednášce sadu tematických původních článků a recentních review.Studenti mají k dispozici aktualizovanou prezentaci k přednášce.

Garant

doc. Mgr. Petr Svoboda, Ph.D.

Vyučující

doc. Mgr. Petr Svoboda, Ph.D.