Předmět The cell nucleus (MB151P100E)

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Materiál	Typ	Datum	Počet stažení

Sylabus

A. Origin and evolution of the cell nucleusLecture 1 - Origin and evolution of the cell nucleusB. Basic concepts and methodological approaches Lecture 2 - Concepts and techniques used in the study of the cell nucleusa. Concepts and modelsb. Electron and light microscopyc. Biochemical approachesC. Constituents and structure of the cell nucleusLecture 3 - Chromatina. The nucleosomeb. The ‘histone code’c. Non-histone proteins and structural RNAsd. Chromatin structure in vivoLecture 4 - The nuclear peripherya. The envelope: interaction with cytoplasmic componentsb. Nuclear poresc. LaminaLecture 5 - Nuclear bodiesa. The nucleolus b. Cajal bodiesc. PML bodiesd. Specklese. ParaspecklesD. The nucleomeLecture 6 - Chromosome territories a. Higher-order organization of chromatinb. Topological domains Lecture 7 - Spatial gene positioning in the nucleusa. The genetic material in three dimensionsb. Impact of positioning on nuclear functionsLecture 8 - DNA replication and DNA repaira. Overview of the replication and repair machineriesb. Spatial and temporal arrangement of DNA replicationc. Preferential sites of DNA repair in the cell nucleusLecture 9 - Gene regulation a. Overview of the transcriptional machineryb. Transcription factoriesc. Dynamics of RNA polymerases and transcription factorsE. The living cell nucleusLecture 10 - Nuclear dynamics a. Self-organization of nuclear compartmentsb. Chromatin mobilityc. mRNA exportLecture 11 - Biophysics of the cell nucleusa. The biophysical properties of chromatinb. Force-generation in the cell nucleusc. Nuclear energetics Lecture 12 - Transcription a. Transcriptional pulsingb. Metabolic regulation of transcription

Literatura

General textbooks Molecular Cell Biology , Lodish et al., 7th edition, 2012, chapters 6-7 Essential Cell Biology , Alberts et al., 4th edition, 2014, chapters 5-8, 18 Cell Biology , Pollard et al., 2nd edition, 2007, chapters 12-14 Specialized books Genome Organization and Function in the Cell Nucleus ,Ed. K. Rippe, Wiley, 2012Scientific articlesReferences to 3-4 exhaustive review articles will be given for each lecture. The students are strongly encouraged to read a selection of these in order to complement the lectures.

Požadavky

At the end of the course, an oral exam will take place, during which 2-3 topics that have been covered during the course will be discussed for a total of 20-30 minutes. Students can choose the language (English or Czech) in which they will pass the oral exam.

Garant

Christian Lanctôt, Dr., Ph.D.