Předmět Biology and Genetics (BIO / ZAA11)

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Materiál	Typ	Datum	Počet stažení

Obsah

Introduction to lab work. Microscopy Techniques. Elementary skills in microscopy, preparation of a specimen. Microscopy Techniques. How to observe stained vs. unstained structures - manipulating the contrast and penetration power. Magnification vs. resolving power. Demonstration of selected techniques. Inoculation of plates. Microorganisms. Evaluation of exposed plates. Bacterial streak plate, yeast streak plate. The epithelial cells colonized by bacteria in mouth cavity. The vital specimen of motile bacterium Clostridium butyricum. Stained preparation of bacterium Escherichia coli. Cell Structure and their Cultivation: Mammalian Cells. Mammalian cells cultures, characterization of cell growth. Cell viability estimation. Semisolid cell cultures; liquid cell cultures. Adherent cell culture. DNA, genetic information, chromosome structure. Proteins. Demonstration: Detection of abnormal hemoglobin on acrylamide gel. Immunodetection of cytoplasmic and nuclear protein, pathological localization of protein. Inclusion bodies of abnormal hemoglobin in red blood cells. Enzymes. Determination of pH optimum for acidic phosphatase. Human amylase activity in a saliva sample. The proof of peroxidase in blood serum. Biomembranes, Osmosis, Homeostasis. Self-assembly of lecithin molecules. Blood cells lysis by detergent. Blood cells in hypertonic solution. Plasmolysis and deplasmolysis. Cell cycle. Mitosis Cell cycle analysis using flow-cytometry, examination of cell nuclei using light microscopy. Cell growth. Mitosis in plant cells. Meiosis. Gametogenesis. Meiosis in locust. Spermatogenesis in human. Blastocyst formation. Cell differentiation, apoptosis. Differentiation of hematopoietic stem cell into blood lineages, blood smears examination. Impaired differentiation in cancer cells. Estimation of caspase activity. Defects in cell differentiation and apoptosis. Blood and Cells of Immune System. Oncogenes, tumor suppressor genes. Individual presentations to the theme. Cell biology.

Získané způsobilosti

The subject allows students to understand elementary cellular processes and rules controlling reproduction of cells and organisms. The knowledge is essential for further study of medicine as well as for clinical practice.

Literatura

Alberts, B. et al. Essential Cell Biology. New York, N.Y. ; London : Garland Science, 2010. ISBN 9780815341291.http://biologie.upol.czMcInnes, Roderick R. Thompson and Thompson genetics in medicine. W. B. Saundesr Company, 2001. ISBN 0721669026.

Požadavky

The attendance of obligatory lab works and seminars in regular or in substitute terms; passed tests; written protocols based on the lab work. Semester credit. Written and oral examination after the second semester of study.

Garant

doc. RNDr. Vladimír Divoký, Ph.D.

Vyučující

doc. RNDr. Vladimír Divoký, Ph.D.RNDr. Ivana Fellnerová, Ph.D.Mgr. Monika Horváthová, Ph.D.doc. RNDr. Petr Mlejnek, Ph.D.Mgr. Jiří Novotný, Ph.D.RNDr. Leona Rašková Kafková, Ph.D.doc. RNDr. Vladimír Divoký, Ph.D.Mgr. Monika Horváthová, Ph.D.doc. RNDr. Petr Mlejnek, Ph.D.Mgr. Jiří Novotný, Ph.D.RNDr. Leona Rašková Kafková, Ph.D.