Předmět Biochemistry 1 (LCH / VAB20)

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Materiál	Typ	Datum	Počet stažení

Obsah

Biochemistry, introduction. Proteins, structure, function. Supramolecular assemblies. Heteroproteins. Structure and function of hemoglobin. Immunoglobulins. Enzymes-structure, classification, mechanism of action. Cofactors and vitamins. Enzyme activity and its regulation. Enzymes in diagnosis. Signal transduction. Mechanism of hormone action. Biochemical function of subcellular organelles and membrane systems. Introduction to metabolic pathways. Bioenergetics. High-energy compounds. Biological oxidation. Respiratory chain, oxidative phosphorylation. Metabolism-overwiev. Citric acid cycle. AcetylCoA-central role in metabolism. Carbohydrate metabolism: overview. Glycolysis. Gluconeogenesis, pentose phosphate pathway, glycogen metabolism. Biomacromolecules in medicineRules of study and directions for laboratory work. Information sources, databases. Laboratory ware. Laboratory techniques. Training of pippeting, weighting, measurement of volume, titration. Manganometric determination of H2O2 in medicinal solution. Determination of water hardness. Preparation of solutions, measurement of pH. Determination of H2SO4 in solution. Preparation, measurement and calculations of pH and buffers capacity. Chemical reactions of amino acids and proteins. Determination of isoelectric points of histidine. Chemical reactions saccharides. Polarimetric observations of D-glucose mutarotation. Chemical reactions of lipids. Determination of the iodine number of lipids. Revision, physical and chemical properties of biologically important compounds. Principles of chromatography. Thin layer chromatography of amino acids. Preparative chromatography of azo dyes on a thin layer. Identification of levomepromazine and its metabolites in biological materials by thin layer chromatography. Ion exchange chromatography - deionization of calcium chloride solution. Gel chromatography of hemolyzed blood. Isolation of plant alkaloids. Principles of electrophoresis. Isolation of albumins and globulins by fraction salting out. Electrophoretic separation of proteins. Separation of acidic, neutral and basic amino acids by electrophoresis. Principles of spectrophotometry. Quantitative determination of salicylates by photometry. Determination of proteins by spectrophotometry (Biuret method). Determination of vitamin C in fruit juices. Determination of nitrates. Practical photometric test. Revision, selected instrumental techniques, chromatography, electrophoresis, spectrophotometry.

Získané způsobilosti

The student who passes the subject will be equipped with theoretical knowledge on basic biochemical pathways and information on regulation of intermediary metabolism. Student will be able to interpret physiological and pathological events in human organism. In practice, student will be able to work according to good laboratory practice rules and to perform basic biochemical tests and to interpret laboratory results.

Literatura

Champe, P. C., Harvey, R. A., Ferrier, D. R. Biochemistry. Lippincott Williams & Wilkins, 2007. ISBN 978-0781769600.Dvořáčková, S. a kol. Biochemistry: laboratory classes. Olomouc: Palacký University, 2007. ISBN 978-80-244-1757-8.Koolman, J., Roehm, K. H. Color Atlas of Biochemistry. Thieme, 2012. ISBN 978-3131003737.Newsholme, E., Leech, A. Functional Biochemistry in Health and Disease. Wiley, 2010. ISBN 978-0-471-98820-5.Murray, R. K., Bender, D. A., Botham, K. M., Kennelly, P. J., Rodwell, V. W., Weil, P. A. Harpers Illustrated Biochemistry. McGraw-Hill, 2012. ISBN 978-0071765763.Devlin, T. M. Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons, 2010. ISBN 978-0470281734.

Požadavky

100 % attendance in practicals

Garant

prof. RNDr. Jitka Ulrichová, CSc.

Vyučující

MUDr. Norbert Cibiček, Ph.D.prof. RNDr. Jitka Ulrichová, CSc.doc. RNDr. Jitka Vostálová, Ph.D.doc. RNDr. Daniela Walterová, CSc.Mgr. Jana Franková, Ph.D.Mgr. Pavel Kosina, Ph.D.doc. Ing. Alena Rajnochová Svobodová, Ph.D.prof. RNDr. Jitka Ulrichová, CSc.doc. Ing. Jan Vacek, Ph.D.Aleksey Zholobenko, Ph.D.