Předmět Biochemistry (LCH / VAB21)

Na serveru studentino.cz naleznete nejrůznější studijní materiály: zápisky z přednášek nebo cvičení, vzorové testy, seminární práce, domácí úkoly a další z předmětu LCH / VAB21 - Biochemistry, Lékařská fakulta, Univerzita Palackého v Olomouci (UP).

Materiál	Typ	Datum	Počet stažení

Obsah

Glycosaminoglycans, proteoglycans. Glycoproteins. Lipid metabolism, overview. Lipoproteins. Fatty acid degradation. Ketone bodies. Fatty acid biosynthesis. Polyunsaturated fatty acids. Eicosanoids. Triacylglycerol metabolism. Lipolysis. Phospholipid and glycolipid metabolism. Cholesterol metabolism. Bile acids. Steroids. Vitamin D. Insulin, glucagon, effect on carbohydrate and lipid metabolism. Pathobiochemistry of carbohydrates. Pathobiochemistry of lipids. Proteolysis, general pathways of amino acids catabolism. Amino acids biosynthesis, urea cycle. Metabolism of aromatic amino acids. Catecholamines. Tetrapyrroles: heme biosynthesis. Bile pigments. Iron metabolism. Nucleotides-structure, function, metabolism. Minerals, trace elements, metabolic and biological role. Biochemical markers in clinical diagnosis. Biologically important radicals. Oxidative stress. Antioxidants. NO - Biosynthesis and function. Gastrointestinal digestion and absorption. Detoxification function of the liver. Metabolism of xenobiotics. Extracellular matrix, Biosynthesis of extracellular proteins. Osteosynthesis. Biochemistry of muscle contraction: energy supply in skeletal and muscle heart. Biochemistry of neurodegenerative diseases. Biochemistry of vision. Nucleic acid, analysis. Cell models in biochemistry and toxicology. Determination of acetylsalicylic acid in Acylpyrin. Enzymes, introduction: nomenclature, structure, factors influencing enzyme activity. Determination of Michaelis constant of alkaline phosphatase (ALP) in serum. Demonstration of enzymatic character of peroxidase. Demonstration of substrate specificity of -amylase and sucrase. Inhibition of enzymatic activity - inhibition of catalase by cyanide. Determination of optimum temperature for trypsin. Enzymes important in diagnostic. Warburgs´ optic test. Determination of lactate dehydrogenase (LD) activity in serum. Determination of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity in serum.Immunoanalytic methods. Determination of rheumatoid factor in serum by LFT. Acido-basic equilibrium. Metabolism of saccharides. Determination of glucose in serum and urine. Oral glucose tolerance test (GTT). Determination of glucose and ketones by diagnostic test strips. Determination of glucose in blood by glucometer. Determination of glycated hemoglobin. Lipid metabolism. Lipid profile: evaluation of diagnostically important parameters in serum (total cholesterol, HDL-cholesterol, triglycerides). Determination of diagnostically important atherosclerosis parameters in serum (total cholesterol, HDL-cholesterol, triglycerides). Amino acids metabolism. Digestion of proteins. Main metabolic pathways of amino acids. Determination of urea in urine and serum. Determination of creatinine in serum and urine; creatinine clearance calculation. Overview of saccharide, lipid and protein metabolism: Course unit test. Microscopy of urinary sediment. Gallstone analysis. Determination of gastric output. Nucleic acids. Nucleotides. Determination of uric acid. Xanthin oxidase. Urinalysis. Tetrapyrrols metabolism. Determination of hemoglobin in blood. Determination of bilirubin in serum. Test for blood in feaces. Test for blood in urine. Practical test: clinical biochemistry methods. Revision, regulation and integration of key metabolic pathways.

Získané způsobilosti

The student who passes the subject will be equipped with theoretical knowledge on basic biochemical pathways and information on regulation of intermediary metabolism. Student will be able to interpret physiological and pathological events in human organism. In practice, student will be able to work according to good laboratory practice rules and to perform basic biochemical tests and to interpret laboratory results.

Literatura

Champe, P. C., Harvey, R. A., Ferrier, D. R. Biochemistry. Lippincott Williams & Wilkins, 2007. ISBN 978-0781769600.Dvořáčková, S. a kol. Biochemistry: laboratory classes. Olomouc: Palacký University, 2007. ISBN 978-80-244-1757-8.Koolman, J., Roehm, K. H. Color Atlas of Biochemistry. Thieme, 2012. ISBN 978-3131003737.Newsholme, E., Leech, A. Functional Biochemistry in Health and Disease. Wiley, 2010. ISBN 978-0-471-98820-5.Murray, R. K., Bender, D. A., Botham, K. M., Kennelly, P. J., Rodwell, V. W., Weil, P. A. Harpers Illustrated Biochemistry. McGraw-Hill, 2012. ISBN 978-0071765763.Devlin, T. M. Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons, 2010. ISBN 978-0470281734.

Požadavky

100 % attendance in practicals

Garant

prof. RNDr. Jitka Ulrichová, CSc.

Vyučující

MUDr. Norbert Cibiček, Ph.D.prof. RNDr. Jitka Ulrichová, CSc.doc. RNDr. Jitka Vostálová, Ph.D.doc. RNDr. Daniela Walterová, CSc.MUDr. Norbert Cibiček, Ph.D.Mgr. Pavel Kosina, Ph.D.doc. Ing. Jan Vacek, Ph.D.doc. RNDr. Jitka Vostálová, Ph.D.doc. RNDr. Daniela Walterová, CSc.