Předmět Immunochemistry (KBBV / C019A)

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Materiál	Typ	Datum	Počet stažení

Obsah

Summary of basic immunology - cells and tissues involved in the immune response, major histocompatibility complex, acquired and specific immune response, humoral and cell-mediated immunity. The basis of clinical immunology focused to the hypersensitivity reactions, tolerance and autoimmunity, organ-specific and systemic autoimmune disease, immunohematology, immune system modulators, immunodeficiencies, infection of the immunocompetent cells, transplantation immunology, tumor immunology and malignancies of the immune system. All above mentioned sections are complemented by applied diagnostic methods.Lectures are completed with the theoretical basis of antigen, antipody structure, and summary of methods for thin isolation and purification, theoretical basis of antigen-antibody interaction (non-covalent bond, affinity and avidity, polyclonal and monoclonal antibodies, genetically engineered antibodies). The immunochemistry part is extended by demostration of methods based on specific Ag-Ab interaction (SDS-PAGE electrophoresis with immunoblotting, immunoelectrophoresis, immunofixation, solid-phase immunoassays - optimization, verification (EIA, RIA, FIA), immunoaffinity chromatography (immunomagnetic separation), isolation and purification of immunoglobulins, antigens. Engineering antibodies. The review of application of immunochemical methods in clinical practice.

Získané způsobilosti

Students know the basics about clinical immunology, diseases of human immune system, also the principle of all the basic tests used in clinical medicine for diagnosis in immunology, microbiology, allergology, transplantation immunology, immunohematology. To predict the complex of diagnostic tests and interpret the results of them.

Literatura

Kay A.B. et al. Allergy and allergic diseases. Blackwell Science, 1997. Phillips T. M. Analytical Techniques in Immunochemistry, Marcel Dekker inc., 1992. Christine D. Stevens. Clinical Immunology and Serology: A Laboratory Perspective, 2nd edition, 2003. Ferencik M. Handbook of Immunochemistry. NY: Chapman & Hall, 1993. Johnstone A., Thorpe R. Immunochemistry in practice, Blackwell Science, 1996. Johnstone Alan P., Turner M. W. Immunochemistry 1-practical approach and Immunochemistry 2-practical approach, Oxford : Oxford University Press, c1997. Virella G. Medical immunology, 5th ed., rev. and expanded, New York : Marcel Dekker, 2001.

Garant

prof. RNDr. Zuzana Bílková, Ph.D.