Předmět Bioengineering (UECHI / C030A)
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Top 10 materiálů tohoto předmětu
Materiály tohoto předmětu
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Další informace
Obsah
Biotechnology - an interdisciplinary pursuit. A contribution of Bioengineering to Biotechnology. What is Bioengineering?Kinetics of enzyme-catalyzed reactions. Simple enzyme kinetics with one and two substrates. Kinetics of substrate utilization, product yield, and biomass production in cell cultures. Overall kinetics in cases of chemical-reaction-mass-transport interaction.Design and analysis of biochemical reactors. The ideal continuous-flow stirred-tank reactor. The ideal plug-flow tubular reactor. Residence-time distributions. Dispersion models for nonideal reactors.Transport phenomena in microbial systems.Bioreactors. Microbial bioreactors. Non-microbial cell culture systems. Enzyme bioreactors. Relationships between batch and continuous biological reactors.General description of stirred vessel bioreactor. Mechanics of fluid flow and gas dispersion in stirred vessel. Experimental design, parameter optimatisation, scaling-up.Bubble column bioreactors. Hydrodynamics. Models of bubble columns. Biochemical loop reactors - propeller (PLR) and air-lift loop reactors (ALR).Bioreactors second generation. Immobilized cell/enzyme bioreactors. Membrane bioreactors.Downstream processing operations. Precipitation. Crystallization. Centrifugation. Membrane separations. Chromatographic methods in bioprocessing. Supercritical fluid extraction.Special problems of bioreactors. Bioreactor and catalyst design for fermentation processes. Costs. Sterilization and preservation in bioprocessing, safety in biotechnology.Fundamentals of computer control in fermentation processes.Biotechnology in waste water treatment and environmental technologies.
Literatura
Doran P.M. Bioprocess Engineering Principles. Academic Press Limited, London, 1995. Ratledge C., Bjorn K. Basic Biotechnology. Cambridge Univ. Press, 2001. Lydersen B.K., D Elia N.A., Nelson K.M. Bioprocess Systems, Equipment and Facilities. John Wiley & Sons, 1994.
Požadavky
závěrečná práce - 60%ústní zkouška - 40%
Garant
prof. Ing. Petr Mikulášek, CSc.