Předmět Laboratory Training in Environmental Technology (S216001)
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Další informace
Sylabus
Option microbiologyThis course will involve 2 blocks of laboratory work:1 Procedures for isoating bacteria with contaminants degrading activity:1 • Isolation of consortia and individual bacteria with degradation abilities from1 • contaminated soil - preparation and dilution of samples, bacteria cultivation,1 • colony counting, Gram staining, microscopy, biochemical characterization of1 • Gram-negative bacteria, identification of bacteria using MALDI-TOF MS, PCR-1 • based detection of functional genes, agarose electrophoresis2 Measurement of toxicity1 • Ecotoxicity tests - as Bioscreen, Vibrio fischeri (luminescent bacteria) - and seed1 • germination will be used for soil ecotoxicity measurements.Option chemical analytical methodsThis course will give introduction into the basic, most common laboratory techniquesused in analysis of drinkingwater, surface water or wastewater. It will involve:1 Introduction into the lab and training on safety rules2 Basic analytical skills (pipetting, weighing, titrations, preparing solutions etc.)3 Gravimetric analytical methods4 Volumetric analytical methods (neutralization capacity, chemical oxygen demand,1 etc.)5 Spectrophotometry (chemical oxygen demand, nitrite, nitrate, etc.)6 Electrochemical methods (pH, redox potential, conductivity, biological oxygen1 demand, etc.)
Literatura
• M. T. Madigan, J. M. Martinko, and J. Parker. Brock Biology of Microorganisms, 11th• Ed. Prentice Hall, 2005.• M.M. Häggblom and I.D. Bossert. Dehalogenation: Microbial Processes and• Environmental Applications. 2003. Kluwer Academic Publishers, London. 2003.• H. L. Ehrlich. Geomicrobiology, 4th Edition, 2004.• A.T. Bull, A.T. Microbial Diversity and Bioprospecting. 2004. ASM Press.• Washington, D.C• E.L. Madsen: Environmental Microbiology. 2008 Blackwell Publishing
Garant
Uhlík Ondřej doc. Ing. Ph.D.Sýkora Vladimír doc. Ing. CSc.